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alpha Fetoprotein anticorps

L’anticorps Souris Monoclonal anti-alpha Fetoprotein a été validé pour IHC et StM. Il convient pour détecter alpha Fetoprotein dans des échantillons de Humain et Souris.
N° du produit ABIN6939240

Aperçu rapide pour alpha Fetoprotein anticorps (ABIN6939240)

Antigène

Voir toutes alpha Fetoprotein (AFP) Anticorps
alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivité

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Humain, Souris

Hôte

  • 157
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Souris

Clonalité

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Monoclonal

Conjugué

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Cet anticorp alpha Fetoprotein est non-conjugé

Application

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Immunohistochemistry (IHC), Staining Methods (StM)

Clone

C2
  • Specificité

    It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

    Aucune reactivité croisée

    Boeuf (Vache), Chien, Rat (Rattus)

    Purification

    Purified by Protein A/G

    Immunogène

    Alpha feto protein (AFP) Purified from serum of a hepatoma patient

    Isotype

    IgG1 kappa
  • Indications d'application

    Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.

    Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions

    For Research Use only
  • Concentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-80 °C

    Stockage commentaire

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Date de péremption

    24 months
  • Antigène

    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

    Autre désignation

    AFP

    Poids moléculaire

    70kDa

    ID gène

    174

    UniProt

    P02771

    Pathways

    C21-Steroid Hormone Metabolic Process
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